abstract：:A mapping population of Paspalum simplex segregating for apomixis (asexual reproduction through seeds) was screened with AFLPs to find apomixis-linked markers. Four AFLPs linked to apomixis in coupling phase were found. Three of them did not show recombinants among the 87 individuals of the mapping population, whereas...

journal_title：Genome

authors： Labombarda P,Busti A,Caceres ME,Pupilli F,Arcioni S

更新日期：2002-06-01 00:00:00

abstract：:DNA gel-blot and in situ hybridization with genome-specific repeated sequences have proven to be valuable tools in analyzing genome structure and relationships in species with complex allopolyploid genomes such as hexaploid oat (Avena sativa L., 2n = 6x = 42; AACCDD genome). In this report, we describe a systematic ap...

journal_title：Genome

authors： Ananiev EV,Vales MI,Phillips RL,Rines HW

更新日期：2002-04-01 00:00:00

abstract：:Whole-genome sequencing is fundamental to understanding the genetic composition of an organism. Given the size and complexity of the soybean genome, an alternative approach is targeted random-gene sequencing, which provides an immediate and productive method of gene discovery. In this study, more than 120000 soybean e...

journal_title：Genome

authors： Shoemaker R,Keim P,Vodkin L,Retzel E,Clifton SW,Waterston R,Smoller D,Coryell V,Khanna A,Erpelding J,Gai X,Brendel V,Raph-Schmidt C,Shoop EG,Vielweber CJ,Schmatz M,Pape D,Bowers Y,Theising B,Martin J,Dante M,Wyl

更新日期：2002-04-01 00:00:00

abstract：:Segregation patterns of polymorphic simple sequence repeat (SSR) primer pairs were investigated in monoploid potato families derived from anther culture. A total of 14 primers developed from the sequences in the database, as well as from a genomic library of potato, was used. Distorted segregation was observed for sev...

journal_title：Genome

authors： Chani E,Ashkenazi V,Hillel J,Veilleux RE

更新日期：2002-04-01 00:00:00

abstract：:Ribosomal intergenic spacers (IGSs) of Odontophrynus americanus 2n and 4n were cloned, restriction mapped, and partially sequenced. Three distinct regions, namely alpha, beta, and delta, were identified in the IGSs. The alpha and beta regions flanked the 28S and 18S rRNA genes, respectively, conserving an identical re...

journal_title：Genome

authors： Alvares LE,Polanco C,Brison O,Coutinho LL,Ruiz IR

更新日期：2002-02-01 00:00:00

abstract：:The brown frog Rana graeca was believed to be present in two areas, the Balkan Peninsula and the Italian Apennines. We have characterised the S1 satellite DNA family from Rana graeca graeca and compared it with that of Rana graeca italica. On Southern blots, the patterns of S1 satellite DNA bands are very different be...

journal_title：Genome

authors： Picariello O,Feliciello I,Bellinello R,Chinali G

更新日期：2002-02-01 00:00:00

abstract：:An improved genetic linkage map has been constructed for cowpea (Vigna unguiculata L. Walp.) based on the segregation of various molecular markers and biological resistance traits in a population of 94 recombinant inbred lines (RILs) derived from the cross between 'IT84S-2049' and '524B'. A set of 242 molecular marker...

journal_title：Genome

authors： Ouédraogo JT,Gowda BS,Jean M,Close TJ,Ehlers JD,Hall AE,Gillaspie AG,Roberts PA,Ismail AM,Bruening G,Gepts P,Timko MP,Belzile FJ

更新日期：2002-02-01 00:00:00

abstract：:Random amplified polymorphic DNA (RAPD) fingerprints of two shrimp populations (Litopenaeus stylirostris) were compared to find genetic marker(s) that may be associated with infectious hypodermal and hematopoietic necrosis virus (IHHNV) resistance or susceptibility. Of the 100 10-mer random primers and 100 intersimple...

journal_title：Genome

authors： Hizer SE,Dhar AK,Klimpel KR,Garcia DK

更新日期：2002-02-01 00:00:00

abstract：:Amplified fragment length polymorphism (AFLP) was used to obtain information on the within-species genetic variability of the tobacco cyst-nematode (TCN) complex. AFLP was found to be well suited to this type of study. The current classification of TCN was confirmed. Results indicate that the Globodera tabacum solanac...

journal_title：Genome

authors： Marché L,Valette S,Grenier E,Mugniéry D

更新日期：2001-12-01 00:00:00

abstract：:Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify ...

journal_title：Genome

authors： Holland JB,Helland SJ,Sharopova N,Rhyne DC

更新日期：2001-12-01 00:00:00

abstract：:Quantitative trait locus (QTL) analysis was used to study the evolution of genes controlling the timing of flowering in four Brassica genomes that are all extensively replicated. Comparative mapping showed that a chromosomal region from the top of Arabidopsis thaliana chromosome 5 corresponded to three homoeologous co...

journal_title：Genome

authors： Axeisson T,Shavorskaya O,Lagercrantz U

更新日期：2001-10-01 00:00:00

abstract：:Microsatellites have many desirable marker properties and have been increasingly used in crop plants in genetic diversity studies. Here we report on the characterisation of microsatellite markers and on their use for the determination of genetic identities and the assessment of genetic variability among accessions fro...

journal_title：Genome

authors： Jakse J,Kindlhofer K,Javornik B

更新日期：2001-10-01 00:00:00

abstract：:Genomic in situ hybridization (GISH) is one of the most popular and effective techniques for detecting alien chromatin introgressed into breeding lines; however, GISH analysis alone does not reveal the genetic identity of the alien chromosomes. We previously isolated a set of bacterial artificial chromosomes (BACs) sp...

journal_title：Genome

authors： Dong F,McGrath JM,Helgeson JP,Jiang J

更新日期：2001-08-01 00:00:00

abstract：:Anther development in angiosperms culminates in the programmed cell death of specific tissues to facilitate the release of pollen. Despite a wealth of morphological descriptions of this process, there have been few reports on the regulation of dehiscence or the coordination of events between tissues. We have cloned an...

journal_title：Genome

authors： Riggs CD,Zeman K,Deguzman R,Rzepczyk A,Taylor AA

更新日期：2001-08-01 00:00:00

abstract：:We are building a framework physical infrastructure across the soybean genome by using SSR (simple sequence repeat) and RFLP (restriction fragment length polymorphism) markers to identify BACs (bacterial artificial chromosomes) from two soybean BAC libraries. The libraries were prepared from two genotypes, each digest...

journal_title：Genome

authors： Marek LF,Mudge J,Darnielle L,Grant D,Hanson N,Paz M,Huihuang Y,Denny R,Larson K,Foster-Hartnett D,Cooper A,Danesh D,Larsen D,Schmidt T,Staggs R,Crow JA,Retzel E,Young ND,Shoemaker RC

更新日期：2001-08-01 00:00:00

abstract：:The results of the development of oil palm (Elaeis guineensis Jacq.) microsatellite markers are given step by step, from the screening of libraries enriched in (GA)n, (GT)n, and (CCG)n simple-sequence repeats (SSRs) to the final characterisation of 21 SSR loci. Also published are primer sequences, estimates of allele ...

journal_title：Genome

authors： Billotte N,Risterucci AM,Barcelos E,Noyer JL,Amblard P,Baurens FC

更新日期：2001-06-01 00:00:00

abstract：:We investigated the 5S ribosomal RNA (rRNA) genes of the isopod crustacean Asellus aquaticus. Using PCR amplification, three different tandemly repeated units containing 5S rDNA were identified. Two of the three sequences were cloned and sequenced. One of them was 1842 bp and presented a 5S rRNA gene and a U1 small nu...

journal_title：Genome

authors： Pelliccia F,Barzotti R,Bucciarelli E,Rocchi A

更新日期：2001-06-01 00:00:00

abstract：:The spectral karyotyping procedure of in situ hybridization with chromosome-specific probes assigns a unique colour code to each of the 21 mouse mitotic chromosomes. We have adapted this procedure to meiotic prophase chromosomes, and the results show that each of the pachytene or metaphase I bivalents can be identifie...

journal_title：Genome

authors： Heng HH,Liu G,Lu W,Bremer S,Ye CJ,Hughes M,Moens P

更新日期：2001-04-01 00:00:00

abstract：:Disease resistance gene candidates (RGCs) belonging to the nucleotide-binding site (NBS) superfamily have been cloned from numerous crop plants using highly conserved DNA sequence motifs. The aims of this research were to (i) isolate genomic DNA clones for RGCs in cultivated sunflower (Helianthus annuus L.) and (ii) m...

journal_title：Genome

authors： Gedil MA,Slabaugh MB,Berry S,Johnson R,Michelmore R,Miller J,Gulya T,Knapp SJ

更新日期：2001-04-01 00:00:00

abstract：:Phytophthora palmivora causes pod rot, a serious disease on cocoa widespread throughout the producing regions. In order to ascertain the genetic determination of cocoa resistance to P. palmivora, a study was carried out on two progenies derived from crosses between a heterozygous, moderately resistant Forastero clone,...

journal_title：Genome

authors： Flament MH,Kebe I,Clément D,Pieretti I,Risterucci AM,N'Goran JA,Cilas C,Despréaux D,Lanaud C

更新日期：2001-02-01 00:00:00

abstract：:The 5S rRNA genes in the Camellia sinensis (L.) O. Kuntze (tea) genome are arranged as tandem repeat units of 300 and 325 bps. The 2 classes of tandem repeats were discovered by Southern hybridisation of tea genomic DNA with a 5S rRNA gene PCR product. ...

journal_title：Genome

authors： Singh D,Singh M

更新日期：2001-02-01 00:00:00

abstract：:Thirty-four polymorphic simple-sequence repeats (SSRs) were evaluated for length polymorphism in melon (Cucumis melo L.) and cucumber (Cucumis sativus L.). SSR markers were located on three melon maps (18 on the map of 'Vedrantais' and PI 161375, 23 on the map of 'Piel de Sapo' and PI 161375, and 16 on the map of PI 4...

journal_title：Genome

authors： Danin-Poleg Y,Reis N,Baudracco-Arnas S,Pitrat M,Staub JE,Oliver M,Arus P,deVicente CM,Katzir N

更新日期：2000-12-01 00:00:00

abstract：:Repetitive DNA sequences have been isolated from a Sau3AI plasmid library of tetraploid Beta corolliflora (2n = 4x = 36), a wild relative of sugar beet (B. vulgaris). The library was screened by differential hybridization with genomic DNA of B. corolliflora and B. vulgaris. When used as probes for Southern hybridizati...

journal_title：Genome

authors： Gao D,Schmidt T,Jung C

更新日期：2000-12-01 00:00:00

abstract：:We have used a PCR and cloning strategy to identify Ty1-copia-like retrotransposons in tomato, Lycopersicon esculentum Mill. Using degenerate oligonucleotide primers corresponding to conserved domains of the Ty1-copia retrotransposon reverse transcriptase (RT), fragments of about 260 bp were obtained by PCR amplificat...

journal_title：Genome

authors： Rogers SA,Pauls KP

更新日期：2000-10-01 00:00:00

abstract：:A novel population consisted of a set of 99 near isogenic lines (NILs) and backcross recombinant inbred lines (BCRILs) derived from a cross between the cultivated tomato Lycopersicon esculentum cv. E6206 and L. hirsutum accession LA1777 is presented. Most of the lines contain a single defined introgression from L. hir...

journal_title：Genome

authors： Monforte AJ,Tanksley SD

更新日期：2000-10-01 00:00:00

abstract：:A model for the genetic structure of grape phylloxera populations in Europe was developed using hierarchical sampling techniques and AFLP-PCR (amplified fragment length polymorphism--polymerase chain reaction) methodology. One-hundred three European and 6 North American phylloxera populations were studied. An addition...

journal_title：Genome

authors： Forneck A,Walker MA,Blaich R

更新日期：2000-08-01 00:00:00

abstract：:As part of a project on lentil molecular genetics, the sequence of the 18S-25S ribosomal RNA gene intergenic spacer (IGS) of Lens culinaris Medik. was determined. DNA was cloned after polymerase chain reaction (PCR) amplification. The spacer of 2939 bp was composed of nonrepetitive sequences and four tandem arrays of ...

journal_title：Genome

authors： Fernández M,Polanco C,Ruiz ML,Pérez de la Vega M

更新日期：2000-08-01 00:00:00

abstract：:Eighteen low-copy and genomic microsatellite markers were tested for Mendelian inheritance and then assayed in 41 Pinus taeda L. samples drawn from five regions in the southern United States. The PCR products had multiple alleles, high levels of polymorphism, and little non-specific priming. Fifteen of the 18 markers ...

journal_title：Genome

authors： Elsik CG,Minihan VT,Hall SE,Scarpa AM,Williams CG

更新日期：2000-06-01 00:00:00

abstract：:The genus Cichorium consists of two widely cultivated species C. intybus (chicory) and C. endivia (endive) and four wild species, C. bottae, C. spinosum, C. calvum, and C. pumilum. A multivariate and an UPGMA (unweighted pair group method average) analysis based on AFLP (amplified fragment length polymorphism) markers...

journal_title：Genome

authors： Kiers AM,Mes TH,van der Meijden R,Bachmann K

更新日期：2000-06-01 00:00:00

abstract：:Intergeneric hybridizations have been made between species of Lolium and Festuca. It has been demonstrated, largely through conventional cytogenetic analysis, that the genomes of the two genera are related, however, much information is lacking on exactly how closely related the genomes are between the two species. We ...

journal_title：Genome

authors： Cao M,Sleper DA,Dong F,Jiang J

更新日期：2000-04-01 00:00:00

abstract：:The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F1-hybrid and its parental lines wa...

journal_title：Genome

authors： Crockett PA,Bhalla PL,Lee CK,Singh MB

更新日期：2000-04-01 00:00:00

abstract：:2B rye plants selected for high (H) or low (L) B transmission rate were studied at pachytene and metaphase I of meiosis to determine the relationship between synapsis, bivalents at metaphase I, and B transmission rate. The results show that the 2 B chromosomes (Bs) form bivalents at pachytene in both the H and L lines...

journal_title：Genome

authors： Jiménez G,Manzanero S,Puertas MJ

更新日期：2000-04-01 00:00:00

abstract：:The wheat homoeologous Group 5 chromosomes were characterized physically in terms of rice linkage blocks using a deletion mapping approach. All three chromosomes, 5A, 5B, and 5D, were shown to have a similar structure, apart from the 4A-5A translocation on the distal end of chromosome arm 5AL. The physical mapping of ...

journal_title：Genome

authors： Sarma RN,Fish L,Gill BS,Snape JW

更新日期：2000-02-01 00:00:00

abstract：:Genomic DNA from 84 individuals of Excoecaria agallocha from seven mangrove populations were analysed for random amplified polymorphic DNAs (RAPDs) using 16 random 10-mer primers. Polymorphism within populations varied from 20% to 31%. At the interpopulation level, 111/149 (74%) of RAPDs were polymorphic. Restriction ...

journal_title：Genome

authors： Lakshmi M,Parani M,Ram N,Parida A

更新日期：2000-02-01 00:00:00

abstract：:We have constructed a tomato genetic linkage map based on an intraspecific cross between two inbred lines of Lycopersicon esculentum and L. esculentum var. cerasiforme. The segregating population was composed of 153 recombinant inbred lines. This map is comprised of one morphological, 132 RFLP (restriction fragment le...

journal_title：Genome

authors： Saliba-Colombani V,Causse M,Gervais L,Philouze J

更新日期：2000-02-01 00:00:00

abstract：:The genomic origin of alien chromosomes present in a wheat-Thinopyrum intermedium partial amphiploid TAF46 (2n = 8x = 56) and six derived chromosome addition lines were analyzed by genomic in situ hybridization (GISH) using S genomic DNA from Pseudoroegneria strigosa (2n = 2x = 14, SS) as a probe. The GISH analysis cl...

journal_title：Genome

authors： Chen Q,Conner RL,Laroche A,Ji WQ,Armstrong KC,Fedak G

更新日期：1999-12-01 00:00:00

abstract：:Southern blots of restriction fragments of genomic DNAs from Hordeum vulgare (L.), H. bulbosum (L.), and interspecific hybrids and their derivatives were hybridized with rDNA probe to identify locus-specific modifications at Rrn loci. H. bulbosum rDNA revealed a single EcoRV site per repeat compared with two sites in ...

journal_title：Genome

authors： Kranthi Kumar P,Subrahmanyam NC

更新日期：1999-12-01 00:00:00

abstract：:We have isolated and characterized microsatellites (simple sequence repeat (SSR) loci) from the silkworm genome. The screening of a partial genomic library by the conventional hybridization method led to the isolation of 28 microsatellites harbouring clones. The abundance of (CA)n repeats in the silkworm genome was ak...

journal_title：Genome

authors： Reddy KD,Abraham EG,Nagaraju J

更新日期：1999-12-01 00:00:00

abstract：:Using contiguous genomic DNA sequences of Arabidopsis thaliana, we were able to identify a region of conserved structure in the genome of rice. The conserved, and presumptive homoeologous segments, are 194 kb and 219-300 kb in size in Arabidopsis and rice, respectively. They contain five homologous genes, distinguishe...

journal_title：Genome

authors： van Dodeweerd AM,Hall CR,Bent EG,Johnson SJ,Bevan MW,Bancroft I

更新日期：1999-10-01 00:00:00

abstract：:Microsatellites or simple sequence repeats (SSRs) were isolated from coconut (Cocos nucifera) and tested for polymorphism on restricted germplasm. Sequencing of 197 clones from a cv. Tagnanan Tall-enriched genomic library showed that 75% contained a microsatellite, of which 64% were dinucleotide (GA/CT, CA/GT and GC/C...

journal_title：Genome

authors： Rivera R,Edwards KJ,Barker JH,Arnold GM,Ayad G,Hodgkin T,Karp A

更新日期：1999-08-01 00:00:00